Isoenzyme analysis
DNA analysis:
• DNA fingerprinting
• Karyology
Expression of desired protein
Peptide sequence of desired protein
Sequence of expression construct
Copy number of expression construct
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Cultivable mycoplasma
Non-cultivable mycoplasma
Infectivity
Electron microscopy
Reverse transcriptase
Other virus specific test
In vitro assay
In vivo assay
Antibody production (MAP, RAP, HAP)
• Karyotyping
• RFLP analysis
• STR analysis
• DNA analysis
Restriction endonuclease mapping
Copy number determination
Coding sequence of the expression construct
Phenotypic
Growth rate
Cellular productivity
Genotypic
DNA & RNA analysis
Biochemically
Product quality
Physically
Functionally
by Saeid
