Livoa LogoLivoa
Gram Staining Procedure
1. Crystal Violet


(Primary Stain - 1 min)

2. Iodine Treatment


(Mordant - 1 min)

3. Decolorization


(Alcohol/Acetone)

Gram-Positive


Remains Purple

Gram-Negative


Becomes Colorless

4. Safranin


(Counterstain - 45 sec)

RESULT: POSITIVE


Purple / Blue

RESULT: NEGATIVE


Pink / Red

Saim

by Saim

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