Sample Prep

Frozen section used with H&E

Preserves original tissue

Antibody Use

Apply tumor-specific antibody (e.g., TTF-1, CDX2)

Identifies cancer type

AC Mixing

AC field agitates antibodies

Fast binding in <20 min

Staining

HRP system produces brown stain

Clear positive/negative result

Interpretation

Pathologist links IHC with H&E

Converts uncertain to certain

Overall Impact

Protein profile in ~20 min

Accuracy ↑ from 85.8% → 94.7%